p70S6K
p70S6K, encoded within the S6K1/RPS6KB1 axis, regulates protein synthesis and cell growth by phosphorylating ribosomal protein S6 after mitogen stimulation[1]. Mechanistically, PI3K/Akt/mTOR signaling activates p70S6K through ordered Ser/Thr phosphorylation, and mTOR-S6K1 dynamics on the eIF3 translation initiation complex connect growth signals to translational control[1][2][3]. In cancer models, enhanced p70S6K expression or activation correlates with poor prognosis and supports epithelial-mesenchymal transition, cancer stemness, and drug resistance[1]. Compared with related isoforms, p85S6K1 contains 23 additional N-terminal amino acids, whereas p70S6K1 is the shorter S6K1 isoform studied mainly in cytoplasmic signaling contexts[5][6]. For experimental applications, rapamycin blocks phosphorylation and activation of 70-kDa S6 kinases, while PF-4708671 provides a cell-permeable S6K1-selective inhibitor for dissecting p70S6K-dependent signaling[4][7].
References:
- [1]. Artemenko M, et al. p70 S6 kinase as a therapeutic target in cancers: More than just an mTOR effector. Cancer Lett. 2022 Jun 1;535:215593. [Content Brief]
- [2]. Holz MK, et al. mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events. Cell. 2005 Nov 18;123(4):569-80. [Content Brief]
- [3]. Chung J, et al. PDGF- and insulin-dependent pp70S6k activation mediated by phosphatidylinositol-3-OH kinase. Nature. 1994 Jul 7;370(6484):71-5. [Content Brief]
- [4]. Chung J, et al. Rapamycin-FKBP specifically blocks growth-dependent activation of and signaling by the 70 kd S6 protein kinases. Cell. 1992 Jun 26;69(7):1227-36. [Content Brief]
- [5]. Grove JR, et al. Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol Cell Biol. 1991;11(11):5541-5550.
- [6]. Kim D, et al. Regulation and localization of ribosomal protein S6 kinase 1 isoforms. Growth Factors. 2009 Feb;27(1):12-21. [Content Brief]
- [7]. Pearce LR, et al. Characterization of PF-4708671, a novel and highly specific inhibitor of p70 ribosomal S6 kinase (S6K1). Biochem J. 2010 Oct 15;431(2):245-55. [Content Brief]
All Product Categories
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p70S6K Related Products (20)
Related Products (20)
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Antibodies (2)
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PF-4708671
0 ImagesPF-4708671 is a potent cell-permeable S6K1 inhibitor with a Ki of 20 nM and IC50 of 160 nM.
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CKI-7 free base
0 ImagesCKI-7 free base is a potent and ATP-competitive casein kinase 1 (CK1) inhibitor with an IC50 of 6 μM and a Ki of 8.5 μM. CKI-7 free base is a selective Cdc7 kinase inhibitor. CKI-7 free base also inhibits SGK, ribosomal S6 kinase-1 (S6K1) and mitogen- and stress-activated protein kinase-1 (MSK1). CKI-7 free base has a much weaker effect on casein kinase II and other protein kinases.
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308-R
0 ImagesCat. No.: HY-151009CAS No.: 2376724-98-8308-R is a SGK3/SGK1/S6K1 inhibitor. 308-R exhibits an IC50 of 5 nM against human SGK3, an IC50 of 10 nM against human SGK1, and an IC50 of 1 nM against human S6K1. 308-R can be used in breast cancer research. It is also applicable to studies related to PROTAC synthesis, such as serving as the target protein ligand for PROTAC SGK3 degrader-2 (HY-176823).
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CKI-7
0 ImagesCKI-7 is a potent and ATP-competitive casein kinase 1 (CK1) inhibitor with an IC50 of 6 μM and a Ki of 8.5 μM. CKI-7 is a selective Cdc7 kinase inhibitor. CKI-7 also inhibits SGK, ribosomal S6 kinase-1 (S6K1) and mitogen- and stress-activated protein kinase-1 (MSK1). CKI-7 has a much weaker effect on casein kinase II and other protein kinases.
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Eudesmin
0 ImagesSynonyms: (-)-Eudesmin; Eudesmine; (-)-EudesmineEudesmin ((-)-Eudesmin) impairs adipogenic differentiation via inhibition of S6K1 signaling pathway. Eudesmin possesses diverse therapeutic effects, including anti-tumor, anti-inflammatory, and anti-bacterial activities.
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LY-2584702 free base
0 ImagesCat. No.: HY-12493CAS No.: 1082949-67-4LY-2584702 free base is a selective ATP competitive inhibitor of p70S6K with an IC50 of 4 nM. In S6K1 enzyme assay, the IC50 of LY-2584702 is 2 nM.
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Hu7691 free base
0 ImagesCat. No.: HY-132302ACAS No.: 2241232-43-7
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HSND80
0 ImagesCat. No.: HY-172392HSND80 (Compound 1) is an orally active inhibitor of MNK/p70S6K, with Kd values of 44 nM against MNK1 and 4 nM against MNK2. HSND80 has a longer target residence time of 45 mins and 58 mins against MNK1 and MNK2 respectively. HSND80 can suppress non-small cell lung cancer (NSCLC) both in vitro and in vivo, and suppress Triple-Negative Breast Cancer (TNBC) in vitro.
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LY-2584702 hydrochloride
0 ImagesCat. No.: HY-12493BCAS No.: 1082948-81-9LY-2584702 hydrochloride is a selective ATP competitive inhibitor of p70S6K with an IC50 of 4 nM. In S6K1 enzyme assay, the IC50 of LY-2584702 is 2 nM.
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